[adegenet-forum] Looking for help with a PCA using adegenet in R
Sarrah Castillo
scastillo at nrdpfc.ca
Tue Oct 19 17:20:29 CEST 2010
Hello Dr. Jombart
I was wondering if you could help me with an issue I am having with your program (Adegenet) in R.
I am attempting to perform a PCA using a structure file. The difference is that this is based on tetraploid data. Structure allows for multiple ploidy, however I am unsure of how to have the program read my data as tetraploid instead of diploid. The genetic information is for a single locus with between 2-4 alleles (with -9 representing missing data)
Here is an example of my file (with -9 representing missing data)
Ind Reg Al1 Al2 Al3 Al4
271 ON 7 10 11 -9
273 ON 2 10 13 -9
272 ON 4 11 12 -9
465 ON 1 2 -9 -9
472 ON 3 6 11 19
489 ON 2 3 4 19
519 ON 7 12 -9 -9
551 ON 7 14 43 -9
466 ON 4 5 15 19
749 ON 7 14 20 26
111 ON 5 7 8 -9
173 ON 4 11 21 -9
318 ON 7 21 24 -9
319 ON 1 20 26 49
323 ON 7 16 20 26
341 ON 7 25 27 49
385 ON 3 19 25 49
485 ON 7 9 12 -9
Ind=individual
Reg=region
Al1= allele 1
Al2= allele 2
Al3= allele 3
Al4= allele 4
Any help would be much appreciated.
Thank you
Sarrah Castillo
____________________
Sarrah Castillo
MSc Candidate
Environmental & Life Sciences Graduate Program
Trent University, 2140 East Bank Drive,
Peterborough, Ontario, K9J 7B8, Canada
e-mail:scastillo at nrdpfc.ca
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