[Seqinr-forum] Need help

[Rinkle Mohapatra]

Dear All
I am working in a project to compare the genomes of  two Vibrio cholerae
pandemic strains ElTor N19661 and  Classical O395. During the course of this
project, I am required to identify genes that are present in the leading and
lagging strands. I have gone through papers and found that identification of
the strands is based on their GC skew. I have interpreted as that when the
GC skew is positive, the corresponding base pair positions are genes coding
from the leading strand. When the GC skew is negative, it corresponds to the
lagging strand. I have considered the graphs that are present in the Oriloc
website for this interpretation. I would be glad if you can tell me whether
this interpretation is correct. I am also interested to know about the role
of the identification of “Origin of Replication” and its role in identifying
the leading and lagging strand.

Best wishes
Rinkle Mohapatra
Banasthali University
India
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