<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=us-ascii"><meta name=Generator content="Microsoft Word 12 (filtered medium)"><style><!--
/* Font Definitions */
@font-face
{font-family:PMingLiU;
panose-1:2 2 3 0 0 0 0 0 0 0;}
@font-face
{font-family:PMingLiU;
panose-1:2 2 3 0 0 0 0 0 0 0;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;}
@font-face
{font-family:PMingLiU;
panose-1:2 2 3 0 0 0 0 0 0 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin-top:0in;
margin-right:0in;
margin-bottom:10.0pt;
margin-left:0in;
line-height:115%;
font-size:11.0pt;
font-family:"Calibri","sans-serif";}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
span.EmailStyle17
{mso-style-type:personal-compose;
font-family:"Calibri","sans-serif";
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.25in 1.0in 1.25in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Dear adegent users and Thibaut,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>I would like to ask questions about using DAPC. </span><span style='font-size:12.0pt;line-height:115%'>I have </span><span style='font-size:12.0pt;line-height:115%'>used </span>import2genind<span style='font-size:12.0pt;line-height:115%'> and </span><span style='font-size:10.0pt;line-height:115%;font-family:"Tahoma","sans-serif";color:black'>df2genind</span><span style='font-size:12.0pt;line-height:115%'> for importing Structure, Genepop, and Fstat files of the same data set to find clusters and to plot Struture-like membership information. Since I was working on a haploid, asexual fungus collected from three regions, I coded data sets as a pseudo-diploid for Genepop and Fstat files but used the original haploid data set for the Structure format. Even though I got K=3 for biological interpretation based on BIC, the membership plot derived from the Structure input file was different from those obtained from the other two file formats. The result using the Structure input format showed many admixture individuals, but the other two input files did not exhibit any admixture individuals (all with membership probability = 1). The latter makes sense to me because the fungus is asexual and genotype flow plays a main role in shaping population structure. Moreover, recombination is not possible for this fungus. Thus, admixture individuals may not occur. What caused this difference between these input files? How do I interpret these results? Thank you for your help.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Regards,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Arhua<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'><o:p> </o:p></span></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>