From nicolas.dussex at gmail.com Fri May 1 00:05:56 2015 From: nicolas.dussex at gmail.com (Nicolas Dussex) Date: Fri, 1 May 2015 10:05:56 +1200 Subject: [adegenet-forum] labeling individuals in scatter pot In-Reply-To: References: Message-ID: Thanks Pablo, seems to be working! Nic On 30 April 2015 at 14:28, pablo fresia wrote: > ... the errors are probably because of the bounds within [..., ...] > > I got this error message now: >> >> > points(dapc1$ind.coord[1:90,], >> + pch=17, cex=1, col="black") >> Error in dapc1$ind.coord[1:90, ] : subscript out of bounds >> > par(xpd=TRUE) >> > points(dapc1$ind.coord[91:218,], >> + pch=18, cex=1.4, col="black") >> Error in dapc1$ind.coord[91:218, ] : subscript out of bounds >> > par(xpd=TRUE) >> > points(dapc1$ind.coord[219:326,], >> + pch=21, cex=1, col="black") >> Error in dapc1$ind.coord[219:326, ] : subscript out of bounds >> > par(xpd=TRUE) >> > points(dapc1$ind.coord[327:392,], >> + pch=20, cex=1.3, col="black") >> Error in dapc1$ind.coord[327:392, ] : subscript out of bounds >> >> I thought about sorting indviduals by morphotype, but not sure how to do >> it as I want to keep the population info too. My samples are grouped by >> population at this stage. >> > > ... mixing both approaches may be you find a way. I figure, if you keep > the inds organized by pop but dividing it by morphotype you can assign > colors and symbols in the way you need. > > eg. pop 1: 1 to 30 inds, 1-15 morph 1 and 16-30 morph 2: > > > points(dapc1$ind.coord[1:15,], pch=17, cex=1, col="black") > > points(dapc1$ind.coord[16:30,], pch=18, cex=1, col="black") > > and so on... > > If I run this: >> >> scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = >> TRUE,scree.da=FALSE,txt.leg = >> c("233","287","418","674","878","957","1085")) #pch=c)#col = myCol) >> par(new=TRUE) >> df <- data.frame(x = dapc1$ind.coord[,1], y = dapc1$ind.coord[,2]) >> morph_data_6m<-read.table("morph_data_6m.txt", header=T) >> c<-morph_data_6m[,1] >> s.label(dfxy = df, xax=1, yax=2, >> label=c,clabel=0.7,boxes=FALSE,grid=FALSE, addaxes=FALSE) >> >> I obtain a plot of coloured dots corresponding to each population and I >> then add the morphotype label (W, WR or X; from my "moprh_data_6m.txt" >> file) on top of each dot, but it gets difficult to read when dots overlap >> (see example attached) >> > > best > pablo > > >> On 30 April 2015 at 13:53, pablo fresia wrote: >> >>> ... maybe, the individuals needs to be by organized morphotype before to >>> run the DAPC. >>> >>> On 29 April 2015 at 22:49, pablo fresia wrote: >>> >>>> Hi Nic, >>>> >>>> an error, should be here >>>> >>>> > myCol <- c("darkblue","darkgreen","red") >>>>> > scatter(dapc1, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, >>>>> cstar=1, clab=1, col=myCol) >>>>> > par(xpd=TRUE) >>>>> > points(*dapc1*$ind.coord[1:90,], >>>>> + pch=17, cex=1, col="black") >>>>> *Error in dapc$ind.coord : object of type 'closure' is not subsettable* >>>>> > par(xpd=TRUE) >>>>> > points(*dapc1*$ind.coord[91:218,], >>>>> + pch=18, cex=1.4, col="black") >>>>> *Error in dapc$ind.coord : object of type 'closure' is not subsettable* >>>>> > par(xpd=TRUE) >>>>> > points(*dapc1*$ind.coord[219:326,], >>>>> + pch=21, cex=1, col="black") >>>>> *Error in dapc$ind.coord : object of type 'closure' is not subsettable* >>>>> > par(xpd=TRUE) >>>>> > points(*dapc1*$ind.coord[327:392,], >>>>> + pch=20, cex=1.3, col="black") >>>>> *Error in dapc$ind.coord : object of type 'closure' is not subsettable* >>>>> >>>> >>>> cheers, >>>> pablo >>>> >>>> >>>>> On 30 April 2015 at 13:03, pablo fresia wrote: >>>>> >>>>>> Hi Nicolas, >>>>>> >>>>>> what's going on, if you try something like this below ? >>>>>> >>>>>> myCol <- c("darkblue","darkgreen","red") >>>>>> scatter(dapc, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, >>>>>> cstar=1, clab=1, col=myCol) >>>>>> par(xpd=TRUE) >>>>>> points(dapc$ind.coord[1:90,], >>>>>> pch=17, cex=1, col="black") >>>>>> par(xpd=TRUE) >>>>>> points(dapc$ind.coord[91:218,], >>>>>> pch=18, cex=1.4, col="black") >>>>>> par(xpd=TRUE) >>>>>> points(dapc$ind.coord[219:326,], >>>>>> pch=21, cex=1, col="black") >>>>>> par(xpd=TRUE) >>>>>> points(dapc$ind.coord[327:392,], >>>>>> pch=20, cex=1.3, col="black") >>>>>> >>>>>> good luck! >>>>>> pablo >>>>>> >>>>>> >>>>>> On 29 April 2015 at 21:50, Nicolas Dussex >>>>>> wrote: >>>>>> >>>>>>> Hi, >>>>>>> >>>>>>> I am doing a DAPC for a dataset that contains 6 populations. Among >>>>>>> all my samples, there are three different morphs and each population >>>>>>> contains a variable mixture of each. What I want to do is to use a >>>>>>> different color for each population (I managed to do it) and use a >>>>>>> different type of point for each sample according to its moprhotype. I have >>>>>>> a file that contains this info ( 1 colomn with either X, W or WR). >>>>>>> >>>>>>> I tried using pch as described below, but the labels do not >>>>>>> correspond to the individuals. >>>>>>> >>>>>>> dapc1 <- dapc(Z1, n.pca = 10, n.da = 3) >>>>>>> c<-morph_data_6m[,1] >>>>>>> scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = >>>>>>> TRUE,scree.da=FALSE,txt.leg = >>>>>>> c("233","287","418","674","878","957","1085"), *pch=c*) >>>>>>> >>>>>>> Would there be a way to assign to each individual a type of point >>>>>>> corresponding to its morphotype? Say point for W, star for X and triangle >>>>>>> for WR. >>>>>>> >>>>>>> Thanks for your help! >>>>>>> Nic >>>>>>> >>>>>>> >>>>>>> -- >>>>>>> Nic Dussex PhD >>>>>>> Department of Zoology >>>>>>> University of Otago >>>>>>> 340 Great King Street >>>>>>> P.O.Box 56 >>>>>>> Dunedin 9054 >>>>>>> New Zealand >>>>>>> >>>>>>> Mobile: +64 21 02790938 >>>>>>> >>>>>>> _______________________________________________ >>>>>>> adegenet-forum mailing list >>>>>>> adegenet-forum at lists.r-forge.r-project.org >>>>>>> >>>>>>> https://lists.r-forge.r-project.org/cgi-bin/mailman/listinfo/adegenet-forum >>>>>>> >>>>>> >>>>>> >>>>> >>>>> >>>>> -- >>>>> Nic Dussex PhD >>>>> Department of Zoology >>>>> University of Otago >>>>> 340 Great King Street >>>>> P.O.Box 56 >>>>> Dunedin 9054 >>>>> New Zealand >>>>> >>>>> Mobile: +64 21 02790938 >>>>> >>>> >>>> >>> >> >> >> -- >> Nic Dussex PhD >> Department of Zoology >> University of Otago >> 340 Great King Street >> P.O.Box 56 >> Dunedin 9054 >> New Zealand >> >> Mobile: +64 21 02790938 >> > > -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -------------- next part -------------- An HTML attachment was scrubbed... URL: From nicolas.dussex at gmail.com Fri May 1 00:48:13 2015 From: nicolas.dussex at gmail.com (Nicolas Dussex) Date: Fri, 1 May 2015 10:48:13 +1200 Subject: [adegenet-forum] labeling individuals in scatter pot In-Reply-To: References: Message-ID: humm.. I realise that it's gonna be difficult to do that with my 300+ samples so if someone has other suggestions, that would be fantastic. Cheers Nic On 1 May 2015 at 10:05, Nicolas Dussex wrote: > Thanks Pablo, seems to be working! > > Nic > > On 30 April 2015 at 14:28, pablo fresia wrote: > >> ... the errors are probably because of the bounds within [..., ...] >> >> I got this error message now: >>> >>> > points(dapc1$ind.coord[1:90,], >>> + pch=17, cex=1, col="black") >>> Error in dapc1$ind.coord[1:90, ] : subscript out of bounds >>> > par(xpd=TRUE) >>> > points(dapc1$ind.coord[91:218,], >>> + pch=18, cex=1.4, col="black") >>> Error in dapc1$ind.coord[91:218, ] : subscript out of bounds >>> > par(xpd=TRUE) >>> > points(dapc1$ind.coord[219:326,], >>> + pch=21, cex=1, col="black") >>> Error in dapc1$ind.coord[219:326, ] : subscript out of bounds >>> > par(xpd=TRUE) >>> > points(dapc1$ind.coord[327:392,], >>> + pch=20, cex=1.3, col="black") >>> Error in dapc1$ind.coord[327:392, ] : subscript out of bounds >>> >>> I thought about sorting indviduals by morphotype, but not sure how to do >>> it as I want to keep the population info too. My samples are grouped by >>> population at this stage. >>> >> >> ... mixing both approaches may be you find a way. I figure, if you keep >> the inds organized by pop but dividing it by morphotype you can assign >> colors and symbols in the way you need. >> >> eg. pop 1: 1 to 30 inds, 1-15 morph 1 and 16-30 morph 2: >> >> > points(dapc1$ind.coord[1:15,], pch=17, cex=1, col="black") >> > points(dapc1$ind.coord[16:30,], pch=18, cex=1, col="black") >> >> and so on... >> >> If I run this: >>> >>> scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = >>> TRUE,scree.da=FALSE,txt.leg = >>> c("233","287","418","674","878","957","1085")) #pch=c)#col = myCol) >>> par(new=TRUE) >>> df <- data.frame(x = dapc1$ind.coord[,1], y = dapc1$ind.coord[,2]) >>> morph_data_6m<-read.table("morph_data_6m.txt", header=T) >>> c<-morph_data_6m[,1] >>> s.label(dfxy = df, xax=1, yax=2, >>> label=c,clabel=0.7,boxes=FALSE,grid=FALSE, addaxes=FALSE) >>> >>> I obtain a plot of coloured dots corresponding to each population and I >>> then add the morphotype label (W, WR or X; from my "moprh_data_6m.txt" >>> file) on top of each dot, but it gets difficult to read when dots overlap >>> (see example attached) >>> >> >> best >> pablo >> >> >>> On 30 April 2015 at 13:53, pablo fresia wrote: >>> >>>> ... maybe, the individuals needs to be by organized morphotype before >>>> to run the DAPC. >>>> >>>> On 29 April 2015 at 22:49, pablo fresia wrote: >>>> >>>>> Hi Nic, >>>>> >>>>> an error, should be here >>>>> >>>>> > myCol <- c("darkblue","darkgreen","red") >>>>>> > scatter(dapc1, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, >>>>>> cstar=1, clab=1, col=myCol) >>>>>> > par(xpd=TRUE) >>>>>> > points(*dapc1*$ind.coord[1:90,], >>>>>> + pch=17, cex=1, col="black") >>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>> subsettable* >>>>>> > par(xpd=TRUE) >>>>>> > points(*dapc1*$ind.coord[91:218,], >>>>>> + pch=18, cex=1.4, col="black") >>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>> subsettable* >>>>>> > par(xpd=TRUE) >>>>>> > points(*dapc1*$ind.coord[219:326,], >>>>>> + pch=21, cex=1, col="black") >>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>> subsettable* >>>>>> > par(xpd=TRUE) >>>>>> > points(*dapc1*$ind.coord[327:392,], >>>>>> + pch=20, cex=1.3, col="black") >>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>> subsettable* >>>>>> >>>>> >>>>> cheers, >>>>> pablo >>>>> >>>>> >>>>>> On 30 April 2015 at 13:03, pablo fresia wrote: >>>>>> >>>>>>> Hi Nicolas, >>>>>>> >>>>>>> what's going on, if you try something like this below ? >>>>>>> >>>>>>> myCol <- c("darkblue","darkgreen","red") >>>>>>> scatter(dapc, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, >>>>>>> cstar=1, clab=1, col=myCol) >>>>>>> par(xpd=TRUE) >>>>>>> points(dapc$ind.coord[1:90,], >>>>>>> pch=17, cex=1, col="black") >>>>>>> par(xpd=TRUE) >>>>>>> points(dapc$ind.coord[91:218,], >>>>>>> pch=18, cex=1.4, col="black") >>>>>>> par(xpd=TRUE) >>>>>>> points(dapc$ind.coord[219:326,], >>>>>>> pch=21, cex=1, col="black") >>>>>>> par(xpd=TRUE) >>>>>>> points(dapc$ind.coord[327:392,], >>>>>>> pch=20, cex=1.3, col="black") >>>>>>> >>>>>>> good luck! >>>>>>> pablo >>>>>>> >>>>>>> >>>>>>> On 29 April 2015 at 21:50, Nicolas Dussex >>>>>>> wrote: >>>>>>> >>>>>>>> Hi, >>>>>>>> >>>>>>>> I am doing a DAPC for a dataset that contains 6 populations. Among >>>>>>>> all my samples, there are three different morphs and each population >>>>>>>> contains a variable mixture of each. What I want to do is to use a >>>>>>>> different color for each population (I managed to do it) and use a >>>>>>>> different type of point for each sample according to its moprhotype. I have >>>>>>>> a file that contains this info ( 1 colomn with either X, W or WR). >>>>>>>> >>>>>>>> I tried using pch as described below, but the labels do not >>>>>>>> correspond to the individuals. >>>>>>>> >>>>>>>> dapc1 <- dapc(Z1, n.pca = 10, n.da = 3) >>>>>>>> c<-morph_data_6m[,1] >>>>>>>> scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = >>>>>>>> TRUE,scree.da=FALSE,txt.leg = >>>>>>>> c("233","287","418","674","878","957","1085"), *pch=c*) >>>>>>>> >>>>>>>> Would there be a way to assign to each individual a type of point >>>>>>>> corresponding to its morphotype? Say point for W, star for X and triangle >>>>>>>> for WR. >>>>>>>> >>>>>>>> Thanks for your help! >>>>>>>> Nic >>>>>>>> >>>>>>>> >>>>>>>> -- >>>>>>>> Nic Dussex PhD >>>>>>>> Department of Zoology >>>>>>>> University of Otago >>>>>>>> 340 Great King Street >>>>>>>> P.O.Box 56 >>>>>>>> Dunedin 9054 >>>>>>>> New Zealand >>>>>>>> >>>>>>>> Mobile: +64 21 02790938 >>>>>>>> >>>>>>>> _______________________________________________ >>>>>>>> adegenet-forum mailing list >>>>>>>> adegenet-forum at lists.r-forge.r-project.org >>>>>>>> >>>>>>>> https://lists.r-forge.r-project.org/cgi-bin/mailman/listinfo/adegenet-forum >>>>>>>> >>>>>>> >>>>>>> >>>>>> >>>>>> >>>>>> -- >>>>>> Nic Dussex PhD >>>>>> Department of Zoology >>>>>> University of Otago >>>>>> 340 Great King Street >>>>>> P.O.Box 56 >>>>>> Dunedin 9054 >>>>>> New Zealand >>>>>> >>>>>> Mobile: +64 21 02790938 >>>>>> >>>>> >>>>> >>>> >>> >>> >>> -- >>> Nic Dussex PhD >>> Department of Zoology >>> University of Otago >>> 340 Great King Street >>> P.O.Box 56 >>> Dunedin 9054 >>> New Zealand >>> >>> Mobile: +64 21 02790938 >>> >> >> > > > -- > Nic Dussex PhD > Department of Zoology > University of Otago > 340 Great King Street > P.O.Box 56 > Dunedin 9054 > New Zealand > > Mobile: +64 21 02790938 > -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -------------- next part -------------- An HTML attachment was scrubbed... URL: From t.jombart at imperial.ac.uk Fri May 1 10:24:51 2015 From: t.jombart at imperial.ac.uk (Jombart, Thibaut) Date: Fri, 1 May 2015 08:24:51 +0000 Subject: [adegenet-forum] labeling individuals in scatter pot In-Reply-To: References: , Message-ID: <2CB2DA8E426F3541AB1907F98ABA6570ABF20E68@icexch-m1.ic.ac.uk> Hi there, I went through the email trail quickly, but I think you need something along the lines of: #### library(adegenet) example(dapc) morpho <- factor(sample(letters[1:6], nrow(dapc1$ind.coord), replace=TRUE)) scatter(dapc1, pch="", scree.da=FALSE) par(xpd=TRUE) points(dapc1$ind.coord, pch=as.integer(morpho)) #### Otherwise ggplot2 offers good alternatives: #### > dat <- data.frame(dapc1$ind.coord, group=factor(dapc1$grp), morpho) > head(dat) LD1 LD2 LD3 LD4 LD5 LD6 AFBIBOR9503 5.398012 -5.9154641 0.30329146 -0.5874714 -0.8290345 -0.26056227 AFBIBOR9504 3.697040 -3.8046570 -0.09263061 -0.5967574 -0.5017393 1.88135969 AFBIBOR9505 5.496883 0.9354478 0.61307612 -0.1419224 -2.6534069 -0.24431807 AFBIBOR9506 5.538440 -2.6152434 0.80186922 0.3334149 -1.6202109 0.06381506 AFBIBOR9507 5.258127 -1.6598447 0.21333638 -1.3799890 -0.1550098 1.77052354 AFBIBOR9508 6.381482 0.5563613 0.42597354 -1.0441841 -1.0207730 -0.04500107 LD7 LD8 LD9 LD10 LD11 AFBIBOR9503 -0.07016765 -1.1810082 -1.0765799 -0.389844937 -0.4576461 AFBIBOR9504 1.68135006 1.5462166 -1.4605117 -0.732729892 -1.5683318 AFBIBOR9505 -0.28373085 0.4224500 -1.5026889 -0.495831033 1.8832038 AFBIBOR9506 0.83341513 -0.857079e-6 -0.4147484 1.304984340 -0.2535325 AFBIBOR9507 -0.23776197 0.3483954 -0.7429532 0.003968628 0.6304628 AFBIBOR9508 0.78436225 -0.2455169 -1.9580097 -0.022258198 -1.0614034 LD12 LD13 LD14 group morpho AFBIBOR9503 -1.1221279 2.0025634 0.2963844 Borgou a AFBIBOR9504 0.4286541 -1.0477615 0.7572506 Borgou d AFBIBOR9505 0.7906680 0.8864259 -2.0664313 Borgou b AFBIBOR9506 -0.9793516 0.8136440 -0.3757589 Borgou c AFBIBOR9507 -1.2099940 -0.0540738 1.3394422 Borgou c AFBIBOR9508 -2.0181906 1.6781495 0.2876485 Borgou c > library(ggplot2) > ggplot(dat, aes(x=LD1, y=LD2)) + geom_point(aes(color=group, shape=morpho)) #### Cheers Thibaut ________________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Nicolas Dussex [nicolas.dussex at gmail.com] Sent: 30 April 2015 23:48 To: pablo fresia; adegenet-forum at lists.r-forge.r-project.org Subject: Re: [adegenet-forum] labeling individuals in scatter pot humm.. I realise that it's gonna be difficult to do that with my 300+ samples so if someone has other suggestions, that would be fantastic. Cheers Nic On 1 May 2015 at 10:05, Nicolas Dussex > wrote: Thanks Pablo, seems to be working! Nic On 30 April 2015 at 14:28, pablo fresia > wrote: ... the errors are probably because of the bounds within [..., ...] I got this error message now: > points(dapc1$ind.coord[1:90,], + pch=17, cex=1, col="black") Error in dapc1$ind.coord[1:90, ] : subscript out of bounds > par(xpd=TRUE) > points(dapc1$ind.coord[91:218,], + pch=18, cex=1.4, col="black") Error in dapc1$ind.coord[91:218, ] : subscript out of bounds > par(xpd=TRUE) > points(dapc1$ind.coord[219:326,], + pch=21, cex=1, col="black") Error in dapc1$ind.coord[219:326, ] : subscript out of bounds > par(xpd=TRUE) > points(dapc1$ind.coord[327:392,], + pch=20, cex=1.3, col="black") Error in dapc1$ind.coord[327:392, ] : subscript out of bounds I thought about sorting indviduals by morphotype, but not sure how to do it as I want to keep the population info too. My samples are grouped by population at this stage. ... mixing both approaches may be you find a way. I figure, if you keep the inds organized by pop but dividing it by morphotype you can assign colors and symbols in the way you need. eg. pop 1: 1 to 30 inds, 1-15 morph 1 and 16-30 morph 2: > points(dapc1$ind.coord[1:15,], pch=17, cex=1, col="black") > points(dapc1$ind.coord[16:30,], pch=18, cex=1, col="black") and so on... If I run this: scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = TRUE,scree.da=FALSE,txt.leg = c("233","287","418","674","878","957","1085")) #pch=c)#col = myCol) par(new=TRUE) df <- data.frame(x = dapc1$ind.coord[,1], y = dapc1$ind.coord[,2]) morph_data_6m<-read.table("morph_data_6m.txt", header=T) c<-morph_data_6m[,1] s.label(dfxy = df, xax=1, yax=2, label=c,clabel=0.7,boxes=FALSE,grid=FALSE, addaxes=FALSE) I obtain a plot of coloured dots corresponding to each population and I then add the morphotype label (W, WR or X; from my "moprh_data_6m.txt" file) on top of each dot, but it gets difficult to read when dots overlap (see example attached) best pablo On 30 April 2015 at 13:53, pablo fresia > wrote: ... maybe, the individuals needs to be by organized morphotype before to run the DAPC. On 29 April 2015 at 22:49, pablo fresia > wrote: Hi Nic, an error, should be here > myCol <- c("darkblue","darkgreen","red") > scatter(dapc1, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, cstar=1, clab=1, col=myCol) > par(xpd=TRUE) > points(dapc1$ind.coord[1:90,], + pch=17, cex=1, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable > par(xpd=TRUE) > points(dapc1$ind.coord[91:218,], + pch=18, cex=1.4, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable > par(xpd=TRUE) > points(dapc1$ind.coord[219:326,], + pch=21, cex=1, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable > par(xpd=TRUE) > points(dapc1$ind.coord[327:392,], + pch=20, cex=1.3, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable cheers, pablo On 30 April 2015 at 13:03, pablo fresia > wrote: Hi Nicolas, what's going on, if you try something like this below ? myCol <- c("darkblue","darkgreen","red") scatter(dapc, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, cstar=1, clab=1, col=myCol) par(xpd=TRUE) points(dapc$ind.coord[1:90,], pch=17, cex=1, col="black") par(xpd=TRUE) points(dapc$ind.coord[91:218,], pch=18, cex=1.4, col="black") par(xpd=TRUE) points(dapc$ind.coord[219:326,], pch=21, cex=1, col="black") par(xpd=TRUE) points(dapc$ind.coord[327:392,], pch=20, cex=1.3, col="black") good luck! pablo On 29 April 2015 at 21:50, Nicolas Dussex > wrote: Hi, I am doing a DAPC for a dataset that contains 6 populations. Among all my samples, there are three different morphs and each population contains a variable mixture of each. What I want to do is to use a different color for each population (I managed to do it) and use a different type of point for each sample according to its moprhotype. I have a file that contains this info ( 1 colomn with either X, W or WR). I tried using pch as described below, but the labels do not correspond to the individuals. dapc1 <- dapc(Z1, n.pca = 10, n.da = 3) c<-morph_data_6m[,1] scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = TRUE,scree.da=FALSE,txt.leg = c("233","287","418","674","878","957","1085"), pch=c) Would there be a way to assign to each individual a type of point corresponding to its morphotype? Say point for W, star for X and triangle for WR. Thanks for your help! Nic -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 _______________________________________________ adegenet-forum mailing list adegenet-forum at lists.r-forge.r-project.org https://lists.r-forge.r-project.org/cgi-bin/mailman/listinfo/adegenet-forum -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -------------- next part -------------- An HTML attachment was scrubbed... URL: From nicolas.dussex at gmail.com Mon May 4 04:52:46 2015 From: nicolas.dussex at gmail.com (Nicolas Dussex) Date: Mon, 4 May 2015 14:52:46 +1200 Subject: [adegenet-forum] labeling individuals in scatter pot In-Reply-To: <2CB2DA8E426F3541AB1907F98ABA6570ABF20E68@icexch-m1.ic.ac.uk> References: <2CB2DA8E426F3541AB1907F98ABA6570ABF20E68@icexch-m1.ic.ac.uk> Message-ID: Hi Thibaut. Thanks for that, I tried something similar to your first example: Z1<-read.genepop("batch_1.genepop_6M.gen",missing=0,quiet=FALSE) dapc1 <- dapc(Z1, n.pca = 15, n.da = 10) scatter(dapc1, pch="", scree.da=FALSE,cell=0, cstar=0,label=NULL, leg = TRUE,txt.leg = c("233","287","418","674","878","957","1085")) par(xpd=TRUE) morph_data_6M<-read.table("morph_data_6M.txt", header=T) morph<-morph_data_6M[,1] points(dapc1$ind.coord, pch=as.integer(morph),col=as.integer(dapc1$grp), lwd=2) It works fine, however I don't really know how to have the colours of my points matching the ones in my lengend, nor how to change the shape of the points. Thanks a lot for your help! Nic On 1 May 2015 at 20:24, Jombart, Thibaut wrote: > > Hi there, > > I went through the email trail quickly, but I think you need something > along the lines of: > > #### > library(adegenet) > example(dapc) > morpho <- factor(sample(letters[1:6], nrow(dapc1$ind.coord), replace=TRUE)) > > scatter(dapc1, pch="", scree.da=FALSE) > par(xpd=TRUE) > points(dapc1$ind.coord, pch=as.integer(morpho)) > > #### > > Otherwise ggplot2 offers good alternatives: > > #### > > dat <- data.frame(dapc1$ind.coord, group=factor(dapc1$grp), morpho) > > head(dat) > LD1 LD2 LD3 LD4 LD5 > LD6 > AFBIBOR9503 5.398012 -5.9154641 0.30329146 -0.5874714 -0.8290345 > -0.26056227 > AFBIBOR9504 3.697040 -3.8046570 -0.09263061 -0.5967574 -0.5017393 > 1.88135969 > AFBIBOR9505 5.496883 0.9354478 0.61307612 -0.1419224 -2.6534069 > -0.24431807 > AFBIBOR9506 5.538440 -2.6152434 0.80186922 0.3334149 -1.6202109 > 0.06381506 > AFBIBOR9507 5.258127 -1.6598447 0.21333638 -1.3799890 -0.1550098 > 1.77052354 > AFBIBOR9508 6.381482 0.5563613 0.42597354 -1.0441841 -1.0207730 > -0.04500107 > LD7 LD8 LD9 LD10 LD11 > AFBIBOR9503 -0.07016765 -1.1810082 -1.0765799 -0.389844937 -0.4576461 > AFBIBOR9504 1.68135006 1.5462166 -1.4605117 -0.732729892 -1.5683318 > AFBIBOR9505 -0.28373085 0.4224500 -1.5026889 -0.495831033 1.8832038 > AFBIBOR9506 0.83341513 -0.857079e-6 -0.4147484 1.304984340 -0.2535325 > AFBIBOR9507 -0.23776197 0.3483954 -0.7429532 0.003968628 0.6304628 > AFBIBOR9508 0.78436225 -0.2455169 -1.9580097 -0.022258198 -1.0614034 > LD12 LD13 LD14 group morpho > AFBIBOR9503 -1.1221279 2.0025634 0.2963844 Borgou a > AFBIBOR9504 0.4286541 -1.0477615 0.7572506 Borgou d > AFBIBOR9505 0.7906680 0.8864259 -2.0664313 Borgou b > AFBIBOR9506 -0.9793516 0.8136440 -0.3757589 Borgou c > AFBIBOR9507 -1.2099940 -0.0540738 1.3394422 Borgou c > AFBIBOR9508 -2.0181906 1.6781495 0.2876485 Borgou c > > library(ggplot2) > > ggplot(dat, aes(x=LD1, y=LD2)) + geom_point(aes(color=group, > shape=morpho)) > > #### > > Cheers > Thibaut > > > ------------------------------ > *From:* adegenet-forum-bounces at lists.r-forge.r-project.org [ > adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Nicolas > Dussex [nicolas.dussex at gmail.com] > *Sent:* 30 April 2015 23:48 > *To:* pablo fresia; adegenet-forum at lists.r-forge.r-project.org > *Subject:* Re: [adegenet-forum] labeling individuals in scatter pot > > humm.. I realise that it's gonna be difficult to do that with my 300+ > samples so if someone has other suggestions, that would be fantastic. > > Cheers > Nic > > On 1 May 2015 at 10:05, Nicolas Dussex wrote: > >> Thanks Pablo, seems to be working! >> >> Nic >> >> On 30 April 2015 at 14:28, pablo fresia wrote: >> >>> ... the errors are probably because of the bounds within [..., ...] >>> >>> I got this error message now: >>>> >>>> > points(dapc1$ind.coord[1:90,], >>>> + pch=17, cex=1, col="black") >>>> Error in dapc1$ind.coord[1:90, ] : subscript out of bounds >>>> > par(xpd=TRUE) >>>> > points(dapc1$ind.coord[91:218,], >>>> + pch=18, cex=1.4, col="black") >>>> Error in dapc1$ind.coord[91:218, ] : subscript out of bounds >>>> > par(xpd=TRUE) >>>> > points(dapc1$ind.coord[219:326,], >>>> + pch=21, cex=1, col="black") >>>> Error in dapc1$ind.coord[219:326, ] : subscript out of bounds >>>> > par(xpd=TRUE) >>>> > points(dapc1$ind.coord[327:392,], >>>> + pch=20, cex=1.3, col="black") >>>> Error in dapc1$ind.coord[327:392, ] : subscript out of bounds >>>> >>>> I thought about sorting indviduals by morphotype, but not sure how to >>>> do it as I want to keep the population info too. My samples are grouped by >>>> population at this stage. >>>> >>> >>> ... mixing both approaches may be you find a way. I figure, if you keep >>> the inds organized by pop but dividing it by morphotype you can assign >>> colors and symbols in the way you need. >>> >>> eg. pop 1: 1 to 30 inds, 1-15 morph 1 and 16-30 morph 2: >>> >>> > points(dapc1$ind.coord[1:15,], pch=17, cex=1, col="black") >>> > points(dapc1$ind.coord[16:30,], pch=18, cex=1, col="black") >>> >>> and so on... >>> >>> If I run this: >>>> >>>> scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = >>>> TRUE,scree.da=FALSE,txt.leg = >>>> c("233","287","418","674","878","957","1085")) #pch=c)#col = myCol) >>>> par(new=TRUE) >>>> df <- data.frame(x = dapc1$ind.coord[,1], y = dapc1$ind.coord[,2]) >>>> morph_data_6m<-read.table("morph_data_6m.txt", header=T) >>>> c<-morph_data_6m[,1] >>>> s.label(dfxy = df, xax=1, yax=2, >>>> label=c,clabel=0.7,boxes=FALSE,grid=FALSE, addaxes=FALSE) >>>> >>>> I obtain a plot of coloured dots corresponding to each population and >>>> I then add the morphotype label (W, WR or X; from my "moprh_data_6m.txt" >>>> file) on top of each dot, but it gets difficult to read when dots overlap >>>> (see example attached) >>>> >>> >>> best >>> pablo >>> >>> >>>> On 30 April 2015 at 13:53, pablo fresia wrote: >>>> >>>>> ... maybe, the individuals needs to be by organized morphotype before >>>>> to run the DAPC. >>>>> >>>>> On 29 April 2015 at 22:49, pablo fresia wrote: >>>>> >>>>>> Hi Nic, >>>>>> >>>>>> an error, should be here >>>>>> >>>>>> > myCol <- c("darkblue","darkgreen","red") >>>>>>> > scatter(dapc1, scree.da=FALSE, bg="white", pch=20, solid=.4, >>>>>>> cex=0, cstar=1, clab=1, col=myCol) >>>>>>> > par(xpd=TRUE) >>>>>>> > points(*dapc1*$ind.coord[1:90,], >>>>>>> + pch=17, cex=1, col="black") >>>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>>> subsettable* >>>>>>> > par(xpd=TRUE) >>>>>>> > points(*dapc1*$ind.coord[91:218,], >>>>>>> + pch=18, cex=1.4, col="black") >>>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>>> subsettable* >>>>>>> > par(xpd=TRUE) >>>>>>> > points(*dapc1*$ind.coord[219:326,], >>>>>>> + pch=21, cex=1, col="black") >>>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>>> subsettable* >>>>>>> > par(xpd=TRUE) >>>>>>> > points(*dapc1*$ind.coord[327:392,], >>>>>>> + pch=20, cex=1.3, col="black") >>>>>>> *Error in dapc$ind.coord : object of type 'closure' is not >>>>>>> subsettable* >>>>>>> >>>>>> >>>>>> cheers, >>>>>> pablo >>>>>> >>>>>> >>>>>>> On 30 April 2015 at 13:03, pablo fresia wrote: >>>>>>> >>>>>>>> Hi Nicolas, >>>>>>>> >>>>>>>> what's going on, if you try something like this below ? >>>>>>>> >>>>>>>> myCol <- c("darkblue","darkgreen","red") >>>>>>>> scatter(dapc, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, >>>>>>>> cstar=1, clab=1, col=myCol) >>>>>>>> par(xpd=TRUE) >>>>>>>> points(dapc$ind.coord[1:90,], >>>>>>>> pch=17, cex=1, col="black") >>>>>>>> par(xpd=TRUE) >>>>>>>> points(dapc$ind.coord[91:218,], >>>>>>>> pch=18, cex=1.4, col="black") >>>>>>>> par(xpd=TRUE) >>>>>>>> points(dapc$ind.coord[219:326,], >>>>>>>> pch=21, cex=1, col="black") >>>>>>>> par(xpd=TRUE) >>>>>>>> points(dapc$ind.coord[327:392,], >>>>>>>> pch=20, cex=1.3, col="black") >>>>>>>> >>>>>>>> good luck! >>>>>>>> pablo >>>>>>>> >>>>>>>> >>>>>>>> On 29 April 2015 at 21:50, Nicolas Dussex < >>>>>>>> nicolas.dussex at gmail.com> wrote: >>>>>>>> >>>>>>>>> Hi, >>>>>>>>> >>>>>>>>> I am doing a DAPC for a dataset that contains 6 populations. >>>>>>>>> Among all my samples, there are three different morphs and each population >>>>>>>>> contains a variable mixture of each. What I want to do is to use a >>>>>>>>> different color for each population (I managed to do it) and use a >>>>>>>>> different type of point for each sample according to its moprhotype. I have >>>>>>>>> a file that contains this info ( 1 colomn with either X, W or WR). >>>>>>>>> >>>>>>>>> I tried using pch as described below, but the labels do not >>>>>>>>> correspond to the individuals. >>>>>>>>> >>>>>>>>> dapc1 <- dapc(Z1, n.pca = 10, n.da = 3) >>>>>>>>> c<-morph_data_6m[,1] >>>>>>>>> scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = >>>>>>>>> TRUE,scree.da=FALSE,txt.leg = >>>>>>>>> c("233","287","418","674","878","957","1085"), *pch=c*) >>>>>>>>> >>>>>>>>> Would there be a way to assign to each individual a type of point >>>>>>>>> corresponding to its morphotype? Say point for W, star for X and triangle >>>>>>>>> for WR. >>>>>>>>> >>>>>>>>> Thanks for your help! >>>>>>>>> Nic >>>>>>>>> >>>>>>>>> >>>>>>>>> -- >>>>>>>>> Nic Dussex PhD >>>>>>>>> Department of Zoology >>>>>>>>> University of Otago >>>>>>>>> 340 Great King Street >>>>>>>>> P.O.Box 56 >>>>>>>>> Dunedin 9054 >>>>>>>>> New Zealand >>>>>>>>> >>>>>>>>> Mobile: +64 21 02790938 >>>>>>>>> >>>>>>>>> _______________________________________________ >>>>>>>>> adegenet-forum mailing list >>>>>>>>> adegenet-forum at lists.r-forge.r-project.org >>>>>>>>> >>>>>>>>> https://lists.r-forge.r-project.org/cgi-bin/mailman/listinfo/adegenet-forum >>>>>>>>> >>>>>>>> >>>>>>>> >>>>>>> >>>>>>> >>>>>>> -- >>>>>>> Nic Dussex PhD >>>>>>> Department of Zoology >>>>>>> University of Otago >>>>>>> 340 Great King Street >>>>>>> P.O.Box 56 >>>>>>> Dunedin 9054 >>>>>>> New Zealand >>>>>>> >>>>>>> Mobile: +64 21 02790938 >>>>>>> >>>>>> >>>>>> >>>>> >>>> >>>> >>>> -- >>>> Nic Dussex PhD >>>> Department of Zoology >>>> University of Otago >>>> 340 Great King Street >>>> P.O.Box 56 >>>> Dunedin 9054 >>>> New Zealand >>>> >>>> Mobile: +64 21 02790938 >>>> >>> >>> >> >> >> -- >> Nic Dussex PhD >> Department of Zoology >> University of Otago >> 340 Great King Street >> P.O.Box 56 >> Dunedin 9054 >> New Zealand >> >> Mobile: +64 21 02790938 >> > > > > -- > Nic Dussex PhD > Department of Zoology > University of Otago > 340 Great King Street > P.O.Box 56 > Dunedin 9054 > New Zealand > > Mobile: +64 21 02790938 > -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -------------- next part -------------- An HTML attachment was scrubbed... URL: From t.jombart at imperial.ac.uk Tue May 5 12:16:36 2015 From: t.jombart at imperial.ac.uk (Jombart, Thibaut) Date: Tue, 5 May 2015 10:16:36 +0000 Subject: [adegenet-forum] labeling individuals in scatter pot In-Reply-To: References: <2CB2DA8E426F3541AB1907F98ABA6570ABF20E68@icexch-m1.ic.ac.uk>, Message-ID: <2CB2DA8E426F3541AB1907F98ABA6570ABF235A9@icexch-m1.ic.ac.uk> Hi there, to specify which pch / color you want to use, define them as a factor, specify the levels corresponding to pch / colour wanted, and then use 'as.character(youFactor)'. Cheers Thibaut ________________________________________ From: Nicolas Dussex [nicolas.dussex at gmail.com] Sent: 04 May 2015 03:52 To: Jombart, Thibaut Cc: pablo fresia; adegenet-forum at lists.r-forge.r-project.org Subject: Re: [adegenet-forum] labeling individuals in scatter pot Hi Thibaut. Thanks for that, I tried something similar to your first example: Z1<-read.genepop("batch_1.genepop_6M.gen",missing=0,quiet=FALSE) dapc1 <- dapc(Z1, n.pca = 15, n.da = 10) scatter(dapc1, pch="", scree.da=FALSE,cell=0, cstar=0,label=NULL, leg = TRUE,txt.leg = c("233","287","418","674","878","957","1085")) par(xpd=TRUE) morph_data_6M<-read.table("morph_data_6M.txt", header=T) morph<-morph_data_6M[,1] points(dapc1$ind.coord, pch=as.integer(morph),col=as.integer(dapc1$grp), lwd=2) It works fine, however I don't really know how to have the colours of my points matching the ones in my lengend, nor how to change the shape of the points. Thanks a lot for your help! Nic On 1 May 2015 at 20:24, Jombart, Thibaut > wrote: Hi there, I went through the email trail quickly, but I think you need something along the lines of: #### library(adegenet) example(dapc) morpho <- factor(sample(letters[1:6], nrow(dapc1$ind.coord), replace=TRUE)) scatter(dapc1, pch="", scree.da=FALSE) par(xpd=TRUE) points(dapc1$ind.coord, pch=as.integer(morpho)) #### Otherwise ggplot2 offers good alternatives: #### > dat <- data.frame(dapc1$ind.coord, group=factor(dapc1$grp), morpho) > head(dat) LD1 LD2 LD3 LD4 LD5 LD6 AFBIBOR9503 5.398012 -5.9154641 0.30329146 -0.5874714 -0.8290345 -0.26056227 AFBIBOR9504 3.697040 -3.8046570 -0.09263061 -0.5967574 -0.5017393 1.88135969 AFBIBOR9505 5.496883 0.9354478 0.61307612 -0.1419224 -2.6534069 -0.24431807 AFBIBOR9506 5.538440 -2.6152434 0.80186922 0.3334149 -1.6202109 0.06381506 AFBIBOR9507 5.258127 -1.6598447 0.21333638 -1.3799890 -0.1550098 1.77052354 AFBIBOR9508 6.381482 0.5563613 0.42597354 -1.0441841 -1.0207730 -0.04500107 LD7 LD8 LD9 LD10 LD11 AFBIBOR9503 -0.07016765 -1.1810082 -1.0765799 -0.389844937 -0.4576461 AFBIBOR9504 1.68135006 1.5462166 -1.4605117 -0.732729892 -1.5683318 AFBIBOR9505 -0.28373085 0.4224500 -1.5026889 -0.495831033 1.8832038 AFBIBOR9506 0.83341513 -0.857079e-6 -0.4147484 1.304984340 -0.2535325 AFBIBOR9507 -0.23776197 0.3483954 -0.7429532 0.003968628 0.6304628 AFBIBOR9508 0.78436225 -0.2455169 -1.9580097 -0.022258198 -1.0614034 LD12 LD13 LD14 group morpho AFBIBOR9503 -1.1221279 2.0025634 0.2963844 Borgou a AFBIBOR9504 0.4286541 -1.0477615 0.7572506 Borgou d AFBIBOR9505 0.7906680 0.8864259 -2.0664313 Borgou b AFBIBOR9506 -0.9793516 0.8136440 -0.3757589 Borgou c AFBIBOR9507 -1.2099940 -0.0540738 1.3394422 Borgou c AFBIBOR9508 -2.0181906 1.6781495 0.2876485 Borgou c > library(ggplot2) > ggplot(dat, aes(x=LD1, y=LD2)) + geom_point(aes(color=group, shape=morpho)) #### Cheers Thibaut ________________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Nicolas Dussex [nicolas.dussex at gmail.com] Sent: 30 April 2015 23:48 To: pablo fresia; adegenet-forum at lists.r-forge.r-project.org Subject: Re: [adegenet-forum] labeling individuals in scatter pot humm.. I realise that it's gonna be difficult to do that with my 300+ samples so if someone has other suggestions, that would be fantastic. Cheers Nic On 1 May 2015 at 10:05, Nicolas Dussex > wrote: Thanks Pablo, seems to be working! Nic On 30 April 2015 at 14:28, pablo fresia > wrote: ... the errors are probably because of the bounds within [..., ...] I got this error message now: > points(dapc1$ind.coord[1:90,], + pch=17, cex=1, col="black") Error in dapc1$ind.coord[1:90, ] : subscript out of bounds > par(xpd=TRUE) > points(dapc1$ind.coord[91:218,], + pch=18, cex=1.4, col="black") Error in dapc1$ind.coord[91:218, ] : subscript out of bounds > par(xpd=TRUE) > points(dapc1$ind.coord[219:326,], + pch=21, cex=1, col="black") Error in dapc1$ind.coord[219:326, ] : subscript out of bounds > par(xpd=TRUE) > points(dapc1$ind.coord[327:392,], + pch=20, cex=1.3, col="black") Error in dapc1$ind.coord[327:392, ] : subscript out of bounds I thought about sorting indviduals by morphotype, but not sure how to do it as I want to keep the population info too. My samples are grouped by population at this stage. ... mixing both approaches may be you find a way. I figure, if you keep the inds organized by pop but dividing it by morphotype you can assign colors and symbols in the way you need. eg. pop 1: 1 to 30 inds, 1-15 morph 1 and 16-30 morph 2: > points(dapc1$ind.coord[1:15,], pch=17, cex=1, col="black") > points(dapc1$ind.coord[16:30,], pch=18, cex=1, col="black") and so on... If I run this: scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = TRUE,scree.da=FALSE,txt.leg = c("233","287","418","674","878","957","1085")) #pch=c)#col = myCol) par(new=TRUE) df <- data.frame(x = dapc1$ind.coord[,1], y = dapc1$ind.coord[,2]) morph_data_6m<-read.table("morph_data_6m.txt", header=T) c<-morph_data_6m[,1] s.label(dfxy = df, xax=1, yax=2, label=c,clabel=0.7,boxes=FALSE,grid=FALSE, addaxes=FALSE) I obtain a plot of coloured dots corresponding to each population and I then add the morphotype label (W, WR or X; from my "moprh_data_6m.txt" file) on top of each dot, but it gets difficult to read when dots overlap (see example attached) best pablo On 30 April 2015 at 13:53, pablo fresia > wrote: ... maybe, the individuals needs to be by organized morphotype before to run the DAPC. On 29 April 2015 at 22:49, pablo fresia > wrote: Hi Nic, an error, should be here > myCol <- c("darkblue","darkgreen","red") > scatter(dapc1, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, cstar=1, clab=1, col=myCol) > par(xpd=TRUE) > points(dapc1$ind.coord[1:90,], + pch=17, cex=1, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable > par(xpd=TRUE) > points(dapc1$ind.coord[91:218,], + pch=18, cex=1.4, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable > par(xpd=TRUE) > points(dapc1$ind.coord[219:326,], + pch=21, cex=1, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable > par(xpd=TRUE) > points(dapc1$ind.coord[327:392,], + pch=20, cex=1.3, col="black") Error in dapc$ind.coord : object of type 'closure' is not subsettable cheers, pablo On 30 April 2015 at 13:03, pablo fresia > wrote: Hi Nicolas, what's going on, if you try something like this below ? myCol <- c("darkblue","darkgreen","red") scatter(dapc, scree.da=FALSE, bg="white", pch=20, solid=.4, cex=0, cstar=1, clab=1, col=myCol) par(xpd=TRUE) points(dapc$ind.coord[1:90,], pch=17, cex=1, col="black") par(xpd=TRUE) points(dapc$ind.coord[91:218,], pch=18, cex=1.4, col="black") par(xpd=TRUE) points(dapc$ind.coord[219:326,], pch=21, cex=1, col="black") par(xpd=TRUE) points(dapc$ind.coord[327:392,], pch=20, cex=1.3, col="black") good luck! pablo On 29 April 2015 at 21:50, Nicolas Dussex > wrote: Hi, I am doing a DAPC for a dataset that contains 6 populations. Among all my samples, there are three different morphs and each population contains a variable mixture of each. What I want to do is to use a different color for each population (I managed to do it) and use a different type of point for each sample according to its moprhotype. I have a file that contains this info ( 1 colomn with either X, W or WR). I tried using pch as described below, but the labels do not correspond to the individuals. dapc1 <- dapc(Z1, n.pca = 10, n.da = 3) c<-morph_data_6m[,1] scatter(dapc1, cstar=0,cex=2.5, cell=0, label=NULL, leg = TRUE,scree.da=FALSE,txt.leg = c("233","287","418","674","878","957","1085"), pch=c) Would there be a way to assign to each individual a type of point corresponding to its morphotype? Say point for W, star for X and triangle for WR. Thanks for your help! Nic -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 _______________________________________________ adegenet-forum mailing list adegenet-forum at lists.r-forge.r-project.org https://lists.r-forge.r-project.org/cgi-bin/mailman/listinfo/adegenet-forum -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 -- Nic Dussex PhD Department of Zoology University of Otago 340 Great King Street P.O.Box 56 Dunedin 9054 New Zealand Mobile: +64 21 02790938 From ferdy44saputra at gmail.com Tue May 19 03:32:57 2015 From: ferdy44saputra at gmail.com (Ferdy Saputra) Date: Tue, 19 May 2015 08:32:57 +0700 Subject: [adegenet-forum] Problem import data on Adegenet Message-ID: Dear all, I'm a new user on Adegenet. I'm sorry to bother you with this question. I have problem to import my data from genepop or fstat format on Adegenet. These errors appear: Error: could not find function "import2genind" Error: could not find function "read.genepop" Error: could not find function "read.fstat" I've tried to import data from nancycats.dat but error. Do you have any advice? Thank you. Best Regards, Ferdy Saputra -------------- next part -------------- An HTML attachment was scrubbed... URL: From t.jombart at imperial.ac.uk Wed May 20 19:24:07 2015 From: t.jombart at imperial.ac.uk (Jombart, Thibaut) Date: Wed, 20 May 2015 17:24:07 +0000 Subject: [adegenet-forum] Problem import data on Adegenet In-Reply-To: References: Message-ID: <2CB2DA8E426F3541AB1907F98ABA6570ABF2F31B@icexch-m1.ic.ac.uk> Hi, did you load the package? Does this work: library("adegenet") example(import2genind) Cheers Thibaut ________________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Ferdy Saputra [ferdy44saputra at gmail.com] Sent: 19 May 2015 02:32 To: adegenet-forum at lists.r-forge.r-project.org Subject: [adegenet-forum] Problem import data on Adegenet Dear all, I'm a new user on Adegenet. I'm sorry to bother you with this question. I have problem to import my data from genepop or fstat format on Adegenet. These errors appear: Error: could not find function "import2genind" Error: could not find function "read.genepop" Error: could not find function "read.fstat" I've tried to import data from nancycats.dat but error. Do you have any advice? Thank you. Best Regards, Ferdy Saputra -------------- next part -------------- An HTML attachment was scrubbed... URL: From ferdy44saputra at gmail.com Thu May 21 05:55:02 2015 From: ferdy44saputra at gmail.com (Ferdy Saputra) Date: Thu, 21 May 2015 10:55:02 +0700 Subject: [adegenet-forum] Problem import data on Adegenet In-Reply-To: <2CB2DA8E426F3541AB1907F98ABA6570ABF2F31B@icexch-m1.ic.ac.uk> References: <2CB2DA8E426F3541AB1907F98ABA6570ABF2F31B@icexch-m1.ic.ac.uk> Message-ID: Dear Dr. Thibaut It's not worked. I use R version: 3.2.0 in windows 8 and 7, and it's still not worked. Do you have any advice? These are errors: > library("adegenet") Loading required package: ade4 Error in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]) : there is no package called ?Rcpp? Error: package or namespace load failed for ?adegenet? > library("adegenet") Error in library("adegenet") : there is no package called ?adegenet? > example(import2genind) Warning message: In example(import2genind) : no help found for ?import2genind? I think I have a problem on R software. I can't load genABEL or other packages. Any suggestions? I'm very sorry to disturb you. Best Regards, Ferdy Saputra Animals Molecular Genetic Laboratory Department of Animal Production and Technology Faculty of Animal Science Bogor Agricultural University -Student- On 21 May 2015 at 00:24, Jombart, Thibaut wrote: > Hi, > > did you load the package? > Does this work: > > library("adegenet") > example(import2genind) > > Cheers > Thibaut > > > > ------------------------------ > *From:* adegenet-forum-bounces at lists.r-forge.r-project.org [ > adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Ferdy > Saputra [ferdy44saputra at gmail.com] > *Sent:* 19 May 2015 02:32 > *To:* adegenet-forum at lists.r-forge.r-project.org > *Subject:* [adegenet-forum] Problem import data on Adegenet > > Dear all, > > I'm a new user on Adegenet. I'm sorry to bother you with this question. I > have problem to import my data from genepop or fstat format on > Adegenet. These errors appear: > > Error: could not find function "import2genind" > Error: could not find function "read.genepop" > Error: could not find function "read.fstat" > > I've tried to import data from nancycats.dat but error. Do you have any > advice? > > Thank you. > > Best Regards, > > Ferdy Saputra > > -------------- next part -------------- An HTML attachment was scrubbed... URL: From Julie.Jacquemin at uni-hohenheim.de Thu May 21 14:09:02 2015 From: Julie.Jacquemin at uni-hohenheim.de (Julie Jacquemin) Date: Thu, 21 May 2015 14:09:02 +0200 Subject: [adegenet-forum] inbreeding coefficient with GBS-SNPs data Message-ID: <555DCADE.8030701@uni-hohenheim.de> Hello, I am trying to calculate inbreeding coefficients for each individual for my different subpopulations. I have thousands of SNPs data, from Genotyping-by-sequencing. I tried to use the function inbreeding in Adegenet but i'm not certain about the results. Here is the command i used and one of my structure file is attached. genind_obj <- read.structure(file="data.stru",n.ind=21,n.loc=6583,onerowperind=FALSE,col.lab=1,col.pop=2,row.marknames=1,NA.char="-9",missing="NA") tempcebe <- inbreeding (genind_obj, N=300) Fmeandistricebe <- sapply(tempcebe, mean) #and to have the mean over the whole subpopulation mean(Fmeandistricebe) My problem is that i obtain, for all my subpopulations (i had three differents) but also when i run it for the global population, always values of F ~ 0,5, for all individuals and for the general mean of course. Also two of my colleagues used the same procedure on their GBS dataset for completely other species, and they also obtain always F very close to 0,5. So i'm thinking they may be an artifact and 0,5 is not the real value i should observe. I also tried with different values for N (nothing changed) and with returning the likelihood function tempcebe <- inbreeding (genind_obj, N=100, res.type="function") And here i only obtained warnings (1: In mean.default(X[[1L]], ...) : argument is not numeric or logical: returning NA 2: In mean.default(X[[2L]], ...) : argument is not numeric or logical: returning NA ...) and only NA values were returned for all individuals. I would be glad to have your comments on this problem. Does this 0,5 recurrent value seem normal? Is the inbreeding function not adapted for NGS data with big number of loci? Or is it a problem with the missing values? Unfortunately with GBS i can not avoid those, i don't have any locus with 0 missing values...Would you suggest anything? Thanks in advance for your help Best Julie  -- Julie Jacquemin, PhD. Post-doc Crop Biodiversity and Breeding Informatics Group (350b) Institute of Plant Breeding, Seed Science and Population Genetics University of Hohenheim Fruwirthstrasse 21, Room 0.25 D-70599 Stuttgart Phone: +49-711-459 24437 jjacquem at uni-hohenheim.de -------------- next part -------------- scaffold_1_37091, scaffold_1_37676, scaffold_1_81876, scaffold_1_82047, scaffold_1_169913, scaffold_1_230821, scaffold_1_230846, scaffold_1_230872, scaffold_1_283264, scaffold_1_391774, scaffold_1_391810, scaffold_1_432154, scaffold_1_480129, scaffold_1_575022, scaffold_1_669126, scaffold_1_669129, scaffold_1_833127, scaffold_1_850054, scaffold_1_918351, scaffold_1_918392, scaffold_1_1337975, scaffold_1_1353001, scaffold_1_1353007, scaffold_1_1353062, scaffold_1_1428178, scaffold_1_1459913, scaffold_1_1530828, scaffold_1_1593727, scaffold_1_1651796, scaffold_1_1713482, scaffold_1_1720035, scaffold_1_1769842, scaffold_1_1769878, scaffold_1_1785032, scaffold_1_1865103, scaffold_1_1865128, 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2 1 4 1 1 1 4 1 1 4 1 2 1 3 2 3 4 1 3 1 4 2 3 4 1 2 1 3 3 2 -9 -9 -9 -9 -9 -9 -9 4 1 3 1 1 2 3 1 1 3 1 3 4 3 4 2 4 1 4 2 3 3 1 2 1 4 4 1 3 4 1 3 3 2 2 3 1 1 4 1 1 1 1 2 1 4 2 3 3 3 4 2 1 2 2 4 3 2 4 1 1 2 1 3 2 4 1 4 1 3 2 3 1 4 3 2 1 3 2 2 3 4 1 1 3 3 1 1 2 3 4 4 1 -9 -9 -9 -9 -9 2 2 2 1 4 4 1 3 1 1 3 3 1 2 2 2 2 1 4 4 2 3 2 2 3 1 4 4 2 -9 -9 -9 -9 -9 -9 -9 -9 3 1 1 1 2 4 3 2 2 3 1 3 1 1 2 3 1 1 1 4 3 2 2 1 3 4 1 2 2 2 -9 -9 -9 -9 -9 1 2 4 1 3 1 1 1 1 1 4 2 3 1 3 3 3 -9 -9 3 4 1 1 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 3 4 3 1 2 2 2 4 2 4 2 4 3 3 1 4 2 1 1 1 2 3 2 1 3 2 3 2 4 3 4 -9 -9 -9 1 1 3 2 1 2 3 4 1 3 2 3 2 1 1 1 1 2 2 2 4 1 -9 4 4 -9 3 -9 4 2 3 2 1 1 1 4 1 3 -9 -9 -9 -9 -9 3 1 1 2 4 2 3 2 1 3 2 3 4 2 4 2 4 3 3 2 3 2 4 1 3 4 2 4 3 4 2 3 4 2 4 4 4 2 2 3 1 3 4 3 1 2 3 1 4 3 2 1 2 1 3 4 3 4 4 1 1 2 1 1 4 1 3 1 2 1 4 1 1 4 4 1 2 1 3 1 1 1 3 2 2 4 2 1 4 3 2 2 1 3 1 3 3 3 2 3 2 1 1 1 3 -9 -9 -9 -9 -9 -9 3 4 2 1 2 4 3 4 1 -9 -9 -9 -9 1 1 -9 3 1 3 -9 -9 4 3 -9 1 3 4 3 3 2 1 2 3 3 3 4 1 3 2 1 2 1 3 1 2 1 1 1 4 1 2 4 -9 -9 1 -9 2 1 4 3 2 4 3 4 -9 -9 4 4 2 2 3 2 1 2 1 2 4 2 -9 3 3 1 3 3 2 -9 4 4 1 3 3 3 4 2 3 3 4 2 2 3 4 3 1 3 2 -9 -9 -9 -9 -9 -9 3 3 4 2 3 3 4 1 2 4 1 1 1 4 -9 -9 -9 -9 -9 4 -9 -9 -9 -9 -9 -9 1 -9 1 1 2 1 3 -9 -9 -9 -9 -9 -9 2 4 3 2 3 3 1 2 2 3 -9 4 2 4 2 4 2 3 2 3 4 -9 4 2 2 -9 2 2 -9 1 4 3 4 1 1 3 -9 -9 -9 2 1 3 3 4 2 -9 -9 -9 3 3 -9 4 -9 2 3 -9 -9 4 2 -9 -9 -9 -9 -9 -9 -9 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 -9 4 3 2 4 1 1 4 1 3 1 2 4 3 2 3 4 3 2 2 4 4 4 -9 -9 -9 -9 2 -9 4 3 3 3 1 4 4 3 3 2 3 3 1 3 -9 1 -9 -9 -9 2 2 3 1 2 3 2 4 4 4 4 -9 3 3 -9 2 2 2 3 4 3 2 4 2 2 4 1 2 2 1 1 1 3 4 3 3 1 3 4 4 3 4 -9 1 1 -9 4 3 2 -9 -9 -9 -9 4 1 1 4 3 3 1 3 1 2 2 2 1 4 1 1 2 3 3 2 3 4 3 1 -9 3 3 -9 -9 1 2 1 1 3 1 3 3 2 1 2 1 1 -9 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 3 -9 3 2 4 2 -9 4 2 4 4 2 4 4 3 1 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 -9 -9 4 4 4 3 2 2 4 -9 1 1 1 2 4 -9 1 -9 -9 3 2 4 3 1 4 1 1 3 2 3 3 1 3 3 2 -9 -9 -9 -9 -9 -9 -9 3 4 3 1 2 2 1 4 1 4 1 2 1 3 2 3 -9 -9 3 1 2 1 2 1 -9 3 2 3 2 -9 1 -9 4 -9 1 3 1 4 4 1 4 1 -9 3 3 -9 -9 -9 2 1 3 4 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 1 2 3 1 1 1 -9 -9 -9 -9 1 1 2 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 2 -9 2 2 2 2 2 1 1 3 4 4 4 3 3 1 1 3 -9 1 -9 -9 -9 -9 1 2 2 4 -9 4 1 3 4 2 1 4 2 -9 -9 -9 -9 -9 -9 -9 -9 3 -9 4 -9 2 2 4 -9 -9 -9 2 3 2 3 2 3 4 1 3 1 -9 2 4 -9 4 3 1 4 1 -9 1 3 -9 -9 4 -9 1 1 2 4 1 2 4 4 -9 -9 -9 -9 -9 -9 1 1 3 1 -9 2 2 1 1 -9 -9 -9 1 4 1 4 4 4 -9 2 -9 -9 -9 2 1 4 1 4 3 3 1 -9 -9 3 -9 1 1 3 2 1 -9 -9 -9 -9 -9 4 3 4 2 1 4 4 1 2 1 4 2 3 1 1 3 2 3 -9 -9 -9 1 4 1 3 4 4 1 2 2 3 3 3 3 4 1 2 3 1 3 1 3 4 3 3 1 2 4 4 -9 4 2 4 -9 -9 -9 3 1 3 1 4 2 4 -9 -9 -9 -9 -9 -9 -9 -9 3 4 4 4 2 -9 3 2 3 4 2 2 2 1 2 2 4 3 3 2 4 4 4 4 2 1 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 4 1 4 4 3 2 2 3 2 4 2 3 1 4 2 1 1 3 3 2 1 1 3 1 1 1 2 4 3 1 1 3 2 4 2 2 1 4 3 3 2 3 3 3 2 3 1 2 1 2 3 1 1 2 -9 -9 4 2 4 3 2 2 3 4 2 3 2 4 2 4 1 2 3 1 2 4 3 -9 -9 -9 -9 -9 -9 -9 1 3 2 3 2 4 2 3 3 3 4 3 3 2 3 2 1 3 3 2 3 2 3 2 3 1 3 3 2 2 1 1 3 2 2 4 2 4 1 2 1 1 2 1 1 3 3 3 4 1 4 1 3 3 3 4 2 4 1 1 1 3 3 3 1 2 1 3 1 4 2 3 4 1 1 1 2 4 3 3 1 1 2 1 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 3 -9 -9 3 3 3 -9 -9 -9 -9 -9 3 2 3 4 2 2 -9 2 2 2 1 3 3 4 3 4 3 1 3 1 -9 3 4 1 4 4 3 1 4 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 3 3 4 4 3 1 1 1 4 1 1 3 3 3 4 -9 1 1 4 1 3 2 1 2 2 3 4 3 4 4 4 1 4 3 4 3 2 4 1 1 1 3 2 4 1 1 4 -9 -9 -9 4 1 1 -9 -9 -9 -9 3 -9 1 3 -9 -9 -9 -9 4 3 4 1 4 3 3 1 2 2 3 1 3 1 1 1 4 -9 -9 4 -9 -9 -9 2 3 1 3 3 -9 -9 1 3 3 2 4 3 4 -9 2 -9 1 2 2 3 4 3 2 2 2 2 2 2 2 -9 -9 -9 1 2 2 2 2 2 -9 3 2 -9 1 3 3 4 3 4 1 3 1 4 2 3 -9 -9 -9 2 2 1 3 2 1 3 4 4 -9 -9 -9 3 2 -9 -9 3 -9 -9 4 2 -9 3 2 1 2 1 -9 -9 2 3 4 2 -9 4 -9 3 3 3 4 1 -9 2 4 1 3 -9 -9 -9 -9 -9 2 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 2 2 1 1 4 2 2 4 -9 -9 -9 3 1 4 -9 4 1 3 1 2 -9 -9 -9 -9 -9 4 -9 2 -9 2 3 2 2 2 1 -9 2 -9 1 3 2 4 3 4 2 4 -9 1 -9 4 3 2 1 -9 -9 -9 2 4 3 -9 -9 -9 -9 -9 -9 -9 -9 3 4 2 4 2 -9 3 -9 4 4 -9 -9 2 2 2 1 1 -9 -9 1 -9 2 3 1 4 1 2 1 3 3 2 1 -9 2 -9 -9 -9 1 1 1 3 4 2 2 4 1 1 -9 1 3 -9 -9 -9 -9 -9 -9 2 -9 -9 -9 -9 -9 -9 2 1 -9 -9 -9 -9 -9 3 2 -9 -9 4 1 2 1 2 2 1 3 1 1 3 -9 -9 -9 4 1 3 1 2 1 3 1 2 2 -9 3 4 1 1 1 -9 -9 1 3 3 3 3 2 1 3 2 3 -9 4 1 3 3 3 1 3 1 4 3 3 4 -9 -9 -9 -9 1 4 -9 -9 -9 -9 -9 -9 -9 2 1 4 4 1 4 3 -9 -9 -9 -9 2 1 1 -9 4 2 -9 -9 -9 -9 -9 -9 -9 -9 4 2 4 -9 -9 -9 2 3 4 2 2 4 4 1 -9 -9 -9 -9 -9 4 -9 -9 2 1 2 1 2 4 2 -9 4 4 3 -9 1 3 1 4 4 4 3 1 1 4 3 2 4 2 -9 -9 2 2 3 2 -9 1 4 3 1 2 3 2 -9 -9 -9 -9 -9 -9 1 1 -9 4 -9 -9 -9 -9 -9 -9 4 3 3 4 4 4 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 3 1 4 1 1 2 1 3 4 -9 3 2 -9 -9 -9 -9 -9 2 2 4 1 4 -9 -9 4 3 3 1 2 3 3 1 4 3 3 2 1 4 2 1 4 4 4 2 4 3 1 1 2 4 1 1 4 3 3 4 2 4 4 4 2 4 4 4 2 3 4 3 2 1 1 4 4 2 1 1 2 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 4 -9 -9 3 4 1 1 2 1 3 1 2 1 3 4 1 -9 -9 -9 -9 -9 -9 -9 -9 -9 2 -9 -9 -9 1 4 4 2 -9 4 1 4 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 2 1 2 4 4 4 2 3 2 1 -9 1 3 -9 3 2 4 2 3 4 3 4 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 -9 -9 2 2 2 3 2 2 2 2 3 1 1 2 2 1 1 4 1 1 4 1 3 4 2 1 2 3 -9 1 3 2 4 3 -9 3 2 1 3 4 4 3 4 4 3 -9 -9 1 3 1 -9 4 2 4 1 1 4 2 1 2 3 4 -9 3 2 1 3 4 1 4 4 1 1 3 -9 2 4 -9 -9 -9 -9 -9 -9 -9 1 -9 -9 -9 -9 -9 -9 -9 3 2 -9 3 4 -9 1 -9 2 4 1 2 3 -9 -9 -9 -9 4 4 2 2 4 3 3 4 3 3 2 1 3 2 4 1 3 -9 2 1 1 2 -9 3 3 2 4 -9 -9 -9 2 4 4 1 4 4 1 4 4 2 2 1 2 -9 -9 4 -9 -9 -9 -9 4 3 4 4 -9 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 2 3 1 -9 -9 -9 -9 2 2 3 -9 -9 3 3 -9 -9 -9 -9 1 1 -9 -9 -9 -9 -9 -9 1 1 3 1 4 1 3 2 1 4 3 2 4 1 4 -9 -9 -9 -9 2 2 2 -9 -9 1 2 2 4 4 1 4 4 3 4 3 -9 -9 -9 -9 1 2 1 -9 -9 -9 -9 2 2 1 2 4 1 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 4 -9 -9 2 1 1 3 1 1 1 2 4 4 4 2 4 4 -9 -9 -9 4 -9 -9 -9 -9 -9 -9 4 3 2 4 4 3 3 4 4 3 -9 -9 -9 1 4 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 3 4 4 4 2 4 1 4 4 3 4 3 1 1 2 1 1 2 4 3 4 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 4 2 -9 -9 -9 -9 4 1 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 1 1 2 3 1 2 1 -9 -9 -9 -9 3 1 2 -9 -9 -9 -9 -9 3 -9 -9 1 4 1 4 3 4 2 4 1 2 2 1 2 4 2 4 3 2 1 3 1 -9 -9 -9 -9 -9 -9 -9 -9 4 2 3 2 4 4 2 2 4 3 3 1 3 1 1 3 1 1 1 3 3 2 3 3 1 3 2 2 3 4 4 2 2 3 4 -9 -9 -9 4 4 4 1 2 2 1 1 2 1 1 4 2 2 2 3 2 2 1 4 4 3 1 4 3 1 4 2 1 3 2 1 4 1 2 2 2 3 1 1 2 3 1 3 1 3 3 3 3 4 3 1 1 1 -9 3 4 1 4 1 2 4 2 4 1 4 -9 4 2 2 3 1 4 -9 1 1 4 3 -9 -9 -9 1 2 3 3 2 3 1 3 2 3 4 1 4 3 4 2 4 -9 -9 -9 3 4 4 -9 -9 -9 2 3 3 3 4 4 1 3 3 2 4 2 2 4 1 2 4 3 3 3 4 2 1 2 4 1 4 3 1 -9 4 3 3 4 3 1 3 1 3 2 3 1 2 4 4 3 3 3 -9 -9 -9 -9 -9 -9 4 4 1 2 4 4 3 4 3 1 3 -9 3 1 4 1 3 1 3 4 -9 -9 3 1 1 4 4 4 1 1 3 4 2 4 1 -9 -9 3 4 1 3 3 2 1 3 4 2 3 2 4 3 4 4 -9 2 1 3 1 2 1 -9 -9 -9 -9 1 1 4 1 1 1 4 -9 -9 -9 4 1 1 3 3 4 4 -9 2 1 1 3 3 -9 -9 -9 4 1 4 -9 1 3 4 2 1 -9 4 -9 2 3 4 3 1 -9 1 1 -9 3 3 2 1 1 1 1 3 3 1 2 1 3 4 3 3 -9 2 2 4 -9 -9 1 2 4 2 3 2 -9 -9 -9 -9 -9 4 3 4 1 1 1 1 -9 -9 -9 -9 4 2 3 1 4 2 4 1 2 1 2 1 4 1 3 4 3 4 4 -9 4 1 1 1 -9 4 1 -9 4 3 3 -9 -9 -9 3 4 -9 3 4 4 -9 -9 4 2 2 4 -9 -9 -9 4 -9 2 1 4 -9 -9 1 4 4 3 -9 -9 -9 -9 -9 4 -9 -9 -9 -9 2 2 2 2 1 -9 -9 -9 3 2 -9 3 2 3 1 4 1 1 4 3 2 -9 -9 2 4 -9 -9 -9 -9 1 3 2 3 2 -9 3 1 -9 -9 -9 -9 -9 4 1 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 3 -9 -9 -9 -9 -9 2 4 -9 2 2 3 4 2 2 1 1 1 3 1 4 1 1 2 -9 3 -9 -9 -9 3 -9 1 1 1 -9 3 2 2 3 -9 -9 1 3 -9 -9 -9 1 -9 -9 -9 -9 -9 2 3 3 -9 -9 2 1 1 4 -9 -9 4 -9 4 -9 -9 -9 2 -9 -9 -9 3 4 -9 -9 -9 -9 -9 4 4 3 1 3 3 4 3 3 4 4 2 3 4 4 4 1 2 2 2 -9 -9 -9 4 3 2 1 2 4 1 -9 3 -9 -9 -9 3 1 2 4 1 1 1 3 2 -9 4 2 1 1 4 3 3 2 4 3 2 1 3 1 3 3 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 -9 4 1 2 3 2 1 4 4 4 -9 2 3 3 2 3 1 -9 2 1 -9 1 3 1 1 From t.jombart at imperial.ac.uk Thu May 21 15:03:46 2015 From: t.jombart at imperial.ac.uk (Jombart, Thibaut) Date: Thu, 21 May 2015 13:03:46 +0000 Subject: [adegenet-forum] Problem import data on Adegenet In-Reply-To: References: <2CB2DA8E426F3541AB1907F98ABA6570ABF2F31B@icexch-m1.ic.ac.uk>, Message-ID: <2CB2DA8E426F3541AB1907F98ABA6570ABF2F43D@icexch-m1.ic.ac.uk> Hi there, yes, it is because the package is not installed. Please check the 'basics' tutorial on the adegenet website: http://adegenet.r-forge.r-project.org/ Cheers Thibaut ============================== Dr Thibaut Jombart MRC Centre for Outbreak Analysis and Modelling Department of Infectious Disease Epidemiology Imperial College - School of Public Health Norfolk Place, London W2 1PG, UK Tel. : 0044 (0)20 7594 3658 http://sites.google.com/site/thibautjombart/ http://sites.google.com/site/therepiproject/ http://adegenet.r-forge.r-project.org/ Twitter: @thibautjombart ________________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Ferdy Saputra [ferdy44saputra at gmail.com] Sent: 21 May 2015 04:55 To: adegenet-forum at lists.r-forge.r-project.org Subject: Re: [adegenet-forum] Problem import data on Adegenet Dear Dr. Thibaut It's not worked. I use R version: 3.2.0 in windows 8 and 7, and it's still not worked. Do you have any advice? These are errors: > library("adegenet") Loading required package: ade4 Error in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]) : there is no package called ?Rcpp? Error: package or namespace load failed for ?adegenet? > library("adegenet") Error in library("adegenet") : there is no package called ?adegenet? > example(import2genind) Warning message: In example(import2genind) : no help found for ?import2genind? I think I have a problem on R software. I can't load genABEL or other packages. Any suggestions? I'm very sorry to disturb you. Best Regards, Ferdy Saputra Animals Molecular Genetic Laboratory Department of Animal Production and Technology Faculty of Animal Science Bogor Agricultural University -Student- On 21 May 2015 at 00:24, Jombart, Thibaut > wrote: Hi, did you load the package? Does this work: library("adegenet") example(import2genind) Cheers Thibaut ________________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Ferdy Saputra [ferdy44saputra at gmail.com] Sent: 19 May 2015 02:32 To: adegenet-forum at lists.r-forge.r-project.org Subject: [adegenet-forum] Problem import data on Adegenet Dear all, I'm a new user on Adegenet. I'm sorry to bother you with this question. I have problem to import my data from genepop or fstat format on Adegenet. These errors appear: Error: could not find function "import2genind" Error: could not find function "read.genepop" Error: could not find function "read.fstat" I've tried to import data from nancycats.dat but error. Do you have any advice? Thank you. Best Regards, Ferdy Saputra -------------- next part -------------- An HTML attachment was scrubbed... URL: From t.jombart at imperial.ac.uk Thu May 21 15:22:14 2015 From: t.jombart at imperial.ac.uk (Jombart, Thibaut) Date: Thu, 21 May 2015 13:22:14 +0000 Subject: [adegenet-forum] inbreeding coefficient with GBS-SNPs data In-Reply-To: <555DCADE.8030701@uni-hohenheim.de> References: <555DCADE.8030701@uni-hohenheim.de> Message-ID: <2CB2DA8E426F3541AB1907F98ABA6570ABF2F49D@icexch-m1.ic.ac.uk> Hi there, sorry but this question is too vague for me to answer properly. If the question is: 'do you think your inbreeding function works', my answer is yes, as it seems to work on data I tested it on. If the question is: 'does it scale well', the answer is not too well. The more loci we have the more we bumped into numerical approximation problems - this issue has been documented on this forum before, worth checking the archives. Thing is, not so many loci are needed to estimate F, and ideally only unliked ones should be kept. The point of 0.5 estimate may be meaningless. If there is no information on inbreeding in the data, you expect a flat distribution for F and 0.5 to be the mean. Is it what you are seeing? Distributions peaking at 0.5 would be a different problem. If you suspect the computations are wrong, please either check the code or come up with an example showing an issue. I will investigate the warning. The example currently fails in adegenet 2.0 and this needs fixing. Cheers Thibaut ============================== Dr Thibaut Jombart MRC Centre for Outbreak Analysis and Modelling Department of Infectious Disease Epidemiology Imperial College - School of Public Health Norfolk Place, London W2 1PG, UK Tel. : 0044 (0)20 7594 3658 http://sites.google.com/site/thibautjombart/ http://sites.google.com/site/therepiproject/ http://adegenet.r-forge.r-project.org/ Twitter: @thibautjombart ________________________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Julie Jacquemin [Julie.Jacquemin at uni-hohenheim.de] Sent: 21 May 2015 13:09 To: adegenet-forum at lists.r-forge.r-project.org Subject: [adegenet-forum] inbreeding coefficient with GBS-SNPs data Hello, I am trying to calculate inbreeding coefficients for each individual for my different subpopulations. I have thousands of SNPs data, from Genotyping-by-sequencing. I tried to use the function inbreeding in Adegenet but i'm not certain about the results. Here is the command i used and one of my structure file is attached. genind_obj <- read.structure(file="data.stru",n.ind=21,n.loc=6583,onerowperind=FALSE,col.lab=1,col.pop=2,row.marknames=1,NA.char="-9",missing="NA") tempcebe <- inbreeding (genind_obj, N=300) Fmeandistricebe <- sapply(tempcebe, mean) #and to have the mean over the whole subpopulation mean(Fmeandistricebe) My problem is that i obtain, for all my subpopulations (i had three differents) but also when i run it for the global population, always values of F ~ 0,5, for all individuals and for the general mean of course. Also two of my colleagues used the same procedure on their GBS dataset for completely other species, and they also obtain always F very close to 0,5. So i'm thinking they may be an artifact and 0,5 is not the real value i should observe. I also tried with different values for N (nothing changed) and with returning the likelihood function tempcebe <- inbreeding (genind_obj, N=100, res.type="function") And here i only obtained warnings (1: In mean.default(X[[1L]], ...) : argument is not numeric or logical: returning NA 2: In mean.default(X[[2L]], ...) : argument is not numeric or logical: returning NA ...) and only NA values were returned for all individuals. I would be glad to have your comments on this problem. Does this 0,5 recurrent value seem normal? Is the inbreeding function not adapted for NGS data with big number of loci? Or is it a problem with the missing values? Unfortunately with GBS i can not avoid those, i don't have any locus with 0 missing values...Would you suggest anything? Thanks in advance for your help Best Julie ? -- Julie Jacquemin, PhD. Post-doc Crop Biodiversity and Breeding Informatics Group (350b) Institute of Plant Breeding, Seed Science and Population Genetics University of Hohenheim Fruwirthstrasse 21, Room 0.25 D-70599 Stuttgart Phone: +49-711-459 24437 jjacquem at uni-hohenheim.de From maeva-angelique.techer at cirad.fr Wed May 27 09:41:38 2015 From: maeva-angelique.techer at cirad.fr (=?utf-8?Q?Ma=C3=A9va_Ang=C3=A9lique?= TECHER) Date: Wed, 27 May 2015 11:41:38 +0400 (RET) Subject: [adegenet-forum] DAPC and morphological data In-Reply-To: <177791313.240521.1432711750602.JavaMail.root@cirad.fr> Message-ID: <375012019.240694.1432712498646.JavaMail.root@cirad.fr> Dear all, I'm trying to perform a DAPC on a morphological dataset which is composed of 176 variables of distances for several populations. The dataset looks like this: > head(jeu) POP X1.2 X1.3 X1.4 X1.5 X1.6 X1.7 X1.8 X1.9 X1.10 X1.11 X1.12 X1.13 X1.14 X1.15 X1.16 X1.17 X1.18 X1.19 X2.3 X2.4 X2.5 X2.6 X2.7 X2.8 X2.9 X2.10 CAF1.1 CAF 0.0837 0.2564 0.3150 0.3667 0.3266 0.2661 0.1942 0.1823 0.1133 0.1667 0.1409 0.2364 0.3959 0.7171 0.5261 0.4894 0.3896 0.4618 0.1742 0.2350 0.2841 0.2773 0.2304 0.1756 0.2089 0.1411 I tried to input the matrix in R using Adegenet and the function df2genind. My problem is that R stopped the importation and posted this message: > morpho<-df2genind(jeu[,1:177], missing = 0, ploidy = 1, type = "codom") Error: C stack usage is too close to the limit Is the problem coming from the variables which are not integers or from the importation script? Thank you very much for your help, Cheers, Ma?va -- Ma?va TECHER Doctorante en G?n?tique des Populations / Ph.D Student in Population Genetics "Diversit? g?n?tique et phylog?ographie de l'abeille Apis mellifera dans la zone Oc?an Indien" UMR PVBMT, Universit? de La R?union - CIRAD 7 chemin de l'IRAT Ligne Paradis, 97410 Saint Pierre, La R?union , Tel: +262 262 49 92 41 Portable: +262 692 05 13 07 maeva-angelique.techer at cirad.fr maeva.techer at hotmail.fr From t.jombart at imperial.ac.uk Fri May 29 12:55:12 2015 From: t.jombart at imperial.ac.uk (Jombart, Thibaut) Date: Fri, 29 May 2015 10:55:12 +0000 Subject: [adegenet-forum] DAPC and morphological data In-Reply-To: <375012019.240694.1432712498646.JavaMail.root@cirad.fr> References: <177791313.240521.1432711750602.JavaMail.root@cirad.fr>, <375012019.240694.1432712498646.JavaMail.root@cirad.fr> Message-ID: <2CB2DA8E426F3541AB1907F98ABA6570ABF300AD@icexch-m1.ic.ac.uk> Hello, your data are not genetic markers, and your input is not compatible at all with what df2genind expects. DAPC is implemented for data.frames and matrices. Here, you should use something along the lines of dapca1 <- dapc(jeu[,-1], grp=jeu[,1]) Cheers Thibaut ______________jeu__________________________ From: adegenet-forum-bounces at lists.r-forge.r-project.org [adegenet-forum-bounces at lists.r-forge.r-project.org] on behalf of Ma?va Ang?lique TECHER [maeva-angelique.techer at cirad.fr] Sent: 27 May 2015 08:41 To: adegenet-forum at lists.r-forge.r-project.org Subject: [adegenet-forum] DAPC and morphological data Dear all, I'm trying to perform a DAPC on a morphological dataset which is composed of 176 variables of distances for several populations. The dataset looks like this: > head(jeu) POP X1.2 X1.3 X1.4 X1.5 X1.6 X1.7 X1.8 X1.9 X1.10 X1.11 X1.12 X1.13 X1.14 X1.15 X1.16 X1.17 X1.18 X1.19 X2.3 X2.4 X2.5 X2.6 X2.7 X2.8 X2.9 X2.10 CAF1.1 CAF 0.0837 0.2564 0.3150 0.3667 0.3266 0.2661 0.1942 0.1823 0.1133 0.1667 0.1409 0.2364 0.3959 0.7171 0.5261 0.4894 0.3896 0.4618 0.1742 0.2350 0.2841 0.2773 0.2304 0.1756 0.2089 0.1411 I tried to input the matrix in R using Adegenet and the function df2genind. My problem is that R stopped the importation and posted this message: > morpho<-df2genind(jeu[,1:177], missing = 0, ploidy = 1, type = "codom") Error: C stack usage is too close to the limit Is the problem coming from the variables which are not integers or from the importation script? Thank you very much for your help, Cheers, Ma?va -- Ma?va TECHER Doctorante en G?n?tique des Populations / Ph.D Student in Population Genetics "Diversit? g?n?tique et phylog?ographie de l'abeille Apis mellifera dans la zone Oc?an Indien" UMR PVBMT, Universit? de La R?union - CIRAD 7 chemin de l'IRAT Ligne Paradis, 97410 Saint Pierre, La R?union , Tel: +262 262 49 92 41 Portable: +262 692 05 13 07 maeva-angelique.techer at cirad.fr maeva.techer at hotmail.fr _______________________________________________ adegenet-forum mailing list adegenet-forum at lists.r-forge.r-project.org https://lists.r-forge.r-project.org/cgi-bin/mailman/listinfo/adegenet-forum From tssfnc at unife.it Fri May 29 12:33:36 2015 From: tssfnc at unife.it (Francesca Tassi) Date: Fri, 29 May 2015 12:33:36 +0200 Subject: [adegenet-forum] DAPC with mtDNA data Message-ID: Hi, I'm trying to do a DAPC with a sequence matrix of mtDNA. Which is the best way to run find.cluster procedure and then DAPC analysis? Many thanks Francesca -- Francesca Tassi, PhD Dipartimento di Scienze della Vita e Biotecnologie Universit? di Ferrara via Borsari 46 I-44121 Ferrara Phone: +39 0532 455951 Fax: +39 0532 249761 -------------- next part -------------- An HTML attachment was scrubbed... URL: